Table 1. The composition of pipette solutions and bath solutions for patch clamp.
Solutions I, II, and VII were adjusted to pH 7.4 with tris, and solutions III, IV, V, VI, and VIII were adjusted to pH 7.2 with tris. BAPTA, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid; ATP, adenosine 5′-triphosphate.
| Solutions (mM) | I | II | III | IV | V | VI | VII | VIII |
|---|---|---|---|---|---|---|---|---|
| NaCl | 140 | 140 | ||||||
| NMDGCl | 150 | 147.7 | ||||||
| KCl | 5 | 5 | 140 | |||||
| CaCl2 | 2 | 2 | 4.1 | |||||
| MgCl2 | 1.2 | 1.2 | 1 | |||||
| Glucose | 10 | 10 | ||||||
| CsCl | 140 | 30 | 140 | |||||
| Cesium aspartate | 110 | |||||||
| MgATP | 3 | 3 | 3 | 3 | ||||
| NaGTP | 0.1 | 0.1 | 0.1 | 0.1 | ||||
| Creatine phosphate disodium | 2.5 | 2.5 | 2.5 | 2.5 | ||||
| EGTA | 0.1 | 0.1 | 0.1 | 10 | ||||
| BAPTA | 5 | |||||||
| Hepes | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |