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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Immunol. 2018 Dec 3;202(1):292–299. doi: 10.4049/jimmunol.1800878

Figure 2. Validation of antibody specificity.

Figure 2.

(A-G1) Wild type BALB/c, SCID, or NSG spleens were probed with antibodies specific for CD3 (PA1–29547; A, B, C), Zap70 (D, E, F), Foxp3 (G, H, I), CD19 (J, K, L), Pax5 (M, N, O), Eomes (P, Q, R), PD1(S, T, U), Granzyme B (V, W, X), Ox40 (Y, Z, A1), CD11b (B1, C1, D1), and CD3 (CD3–12; E1, F1, G1). For detection, Warp Red chromogenic substrate was used. Slides were counterstained with hematoxylin and scanned at 20X using Hamamatsu NanoZoomer 2.0-HT (H1-S1) Livers from control- or clodronate-liposome treated BALB/c mice were stained for F4/80 (D2S9R and SP115; H1, I1 and J1, K1, respectively), Fcgr1 (L1, M1), Fcgr4 (N1, O1), CD163 (P1, Q1), or secondary-only (R1, S1). Slides were scanned at 20X using the Hamamatsu NanoZoomer 2.0-HT. All images represent a 40X field-of-view. Scale bar = 100 μm for all images.