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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Immunol. 2018 Nov 30;202(1):105–118. doi: 10.4049/jimmunol.1800598

Figure 4: CCR2 is Functional and Required for Decrease in IFN-α.

Figure 4:

(A) Flow cytometry was performed on MDM using antibodies specific to CCR2 or an isotype matched negative control. After subtracting the contribution from the isotype matched control, the median fluorescence intensity (MFI) of CCR2 from 10 donors was determined. (B) MDM from eight donors were loaded with a calcium sensitive dye and flow cytometric determination of calcium influx was determined. Arrow indicates CCL2 addition. (C) MDM from nine donors were treated with CCL2 following pretreatment with the CCR2 inhibitor propagermanium or vehicle and qRT-PCR performed to quantitate IFN-α.