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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Arthritis Rheumatol. 2018 Dec 4;71(1):109–120. doi: 10.1002/art.40672

Figure 6. MIF has a robust quantitative relationship with CD74, CD44, and CD44 at the single cell level in monocytes stimulated with the lupus snRNP immune complex.

Figure 6

Monocytes purified from healthy donors were incubated for 5 hours with or without U1-snRNP (snRNP, 5 μg/ml) and anti-U1-snRNP antibody-positive (Ab+) serum (5% final concentration) (referred to as immune complex, IC) in the presence or absence of the MIF antagonist MIF098 (20 μM), stained with a set of antibodies, and run on a Helios CyTOF as in Fig 5. The computational algorithms conditional-Density Resampled Estimate of Mutual Information (DREMI) and conditional-Density Rescaled Visualization (DREVI) were performed on the incubated monocytes. DREVI plots show the quantitative relationship of MIF with CD74 (A), CD44 (B), CXCR4 (C), and CXCR2 (D). DREMI scores indicating the strength of the statistical dependency between two molecules are shown above the DREVI plots. Representative data from 4 independent experiments with 4 donors.