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Current methods to increase solubility of insulin-based peptides. (A) Liu et al. Isoacyl peptide method using a threonine and serine to perform O-to-N acyl shift. (B) Hossain et al. a C-terminus 4-hydroxymethoxybenzoic linker with pentalysine tag. (C) Our method using a N-terminus Fmoc-Ddae-OH linker with trilysine tag.
