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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Immunol. 2018 Dec 10;202(1):228–238. doi: 10.4049/jimmunol.1800847

Figure 5.

Figure 5.

SLAMF7 activation on monocytes is selective for alpha chemokines and may not be mediated by any of the inhibitory phosphatases known to interact with SLAMF7. (A and B) We assessed the effects of SLAMF7-mediated inhibition of other alpha chemokines (A) and ISGs (B) in isolated monocytes from HCs at the mRNA level by qRT-PCR. (C) The effects of SHP1/2 and SHIP1 on SLAMF7-mediated inhibition of CXCL10 was assessed via small molecule inhibitors (SHP1/2: NSC87877, 10μM) (SHIP1: 3AC, 5μM). SHP1/2 effects on alpha chemokines were also assessed at the mRNA level (A). (D) The role of CD45 in SLAMF7-mediated inhibition of CXCL10 was assessed via a small molecule inhibitor (CAS 345630–40-2, 1μM). (A and B) representative of 4 independent experiments. Groups in (A, B, C, and D) were compared using 1-way ANOVA with Tukey’s multiple comparison test. Data presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.