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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Dev Biol. 2018 Jun 27;444(Suppl 1):S237–S251. doi: 10.1016/j.ydbio.2018.06.018

Figure 5. Cad6B NTFs do not induce MMP2 levels through transcriptional regulation in cranial neural crest cells in vivo.

Figure 5.

(A) Embryos were unilaterally electroporated with GFP (black) or L-NTF (gray) DNA expression constructs at the 3ss and incubated for an additional 5 hours when neural crest cells are undergoing EMT en masse and dorsal neural tube basement membrane is being degraded (6–7ss). Excised neural crest cells (five embryos per replicate) were pooled and lysed for RNA extraction, followed by generation of cDNA. (A) Electroporation efficiency and relative expression levels of L-NTF were assessed using primers designed to amplify L-NTF. (B) Gene levels were normalized to 18S ribosomal RNA, and the graph shows differences in gene expression as determined by calculating the fold-change from the control group (GFP; arbitrarily set to 1; n = 3).