Table 1.
Molecular Weight in kDa (Mean) | |||||
---|---|---|---|---|---|
Experiments (n) | PNGase F − | PNGase F + | |||
Protein | Source | (untreated) | (treated) | Difference | |
Full-length Cad6B ( # in Fig. 2) |
In vivo | 2 | 122 | 106 | 16 |
In vitro | 1 | 135 | 113 | 22 | |
In vivo | 2 | 95 | 80 | 15 | |
Cad6B L-NTF ( + in Fig. 2) |
Rec. L-NTF-HA (In vivo) | 1 | 84 | 74 | 10 |
In vitro | 4 | 88 | 71 | 17 | |
Rec. L-NTF (In vitro) | 1 | 92 | 73 | 19 | |
Rec. L-NTF-HA (In vitro) | 1 | 93 | 72 | 21 | |
In vivo | 1 | 83 | 70 | 13 | |
Cad6B S-NTF ( * in Fig. 2) |
Rec. S-NTF-HA (In vivo) | 1 | 70 | 61 | 9 |
In vitro | 3 | 70 | 56 | 14 | |
Rec. S-NTF (In vitro) | 1 | 71 | 59 | 12 | |
Rec. S-NTF-HA (In vitro) | 1 | 72 | 62 | 10 |
All analyses were performed on 7−10% SDS-PAGE gels.