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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Cell Rep. 2017 Sep 5;20(10):2455–2467. doi: 10.1016/j.celrep.2017.08.049

Figure 3. Heparin Increases AgRP Signal In Vivo.

Figure 3.

(A and C) Representative images (A) and quantification (C) of c-Fos expression in the ARH of NPY-GFP mice 3 hr after i.p injection of 1 mg/kg heparin or saline.

(B and D) Representative images (B) and quantification (D) of c-Fos expression in the ARH of NPY-GFP mice 3 hr after i.p injection of 5 U/kg heparinase or saline.

(E) Immunoblots and quantification of AgRP and POMC protein expression in the hypothalamus of male C57BL6/J mice 3 hr after i.p injection of 1 mg/kg heparin or saline.

Results are presented as mean ± SEM. **p ≤ 0.01 and ***p ≤ 0.001 by non-paired Student’s t test.