KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-KDM5B | Sigma-Aldrich | Cat# HPA027179; RRID: AB_1851987 |
| Rabbit polyclonal anti-KDM5B | Novus | Cat# 22260002; RRID: AB_10004656 |
| Mouse monoclonal anti-H3K4me3 | Abcam | Cat# ab1012; RRID: AB_442796 |
| Rabbit polyclonal anti-H3K4me2 | Millipore | Cat# 07–030; RRID: AB_10099880 |
| Rabbit polyclonal anti-H3K4me1 | Abcam | Cat# ab8895; RRID: AB_306847 |
| Rabbit polyclonal anti-Histone H3 | Abcam | Cat# ab1791; RRID: AB_302613 |
| Mouse monoclonal anti-beta-Actin | Sigma-Aldrich | Cat# A2228; RRID: AB_476697 |
| Rabbit polyclonal anti-H3K27Ac | Abcam | Cat# ab4729; RRID: AB_2118291 |
| Mouse monoclonal anti-H3K27me3 | Abcam | Cat# ab6002; RRID: AB_305237 |
| Rabbit polyclonal anti-H3K27me2 | Abcam | Cat# ab24684; RRID: AB_448222 |
| Rabbit polyclonal anti-H3K36me2 | Abcam | Cat# ab9049; RRID: AB_1280939 |
| Rabbit polyclonal anti-H3K9Ac | Abcam | Cat# ab4441; RRID: AB_2118292 |
| Rabbit polyclonal anti-H3K79me2 | Abcam | Cat# ab3594; RRID: AB_303937 |
| Rabbit monoclonal anti-SUZ12 | Cell Signaling Technology | Cat# 3737; RRID: AB_2196850 |
| Rabbit monoclonal anti-ERα | Cell Signaling Technology | Cat# 8644; RRID: AB_2617128 |
| Mouse monoclonal anti-phospho-ERα Ser118 | Cell Signaling Technology | Cat# 2511; RRID: AB_331289 |
| Rabbit polyclonal anti-Cleaved Caspase-3 (Asp175) | Cell Signaling Technology | Cat# 9661; RRID: AB_2341188 |
| Rabbit polyclonal anti-Histone H3 (phospho S10) | Abcam | Cat# ab5176; RRID: AB_304763 |
| Rabbit polyclonal anti-H3K4me3 | Abcam | Cat# 8580; RRID: AB_306649 |
| Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat# A-11034; RRID: AB_2576217 |
| Antibodies used for CYTOF (metal) | ||
| Rabbit monoclonal anti-PR a/b (141Pr) | Cell Signaling Technology | Cat# 8757 |
| Mouse monoclonal anti-CD10 (142Nd) | BD Biosciences | Cat# 555373; RRID: AB_395775 |
| Rat monoclonal anti-CD44 (143Nd) | Biolegend | Cat# 103002; RRID: AB_312953 |
| Mouse monoclonal anti-cyclin D3 (144Nd) | Abcam | Cat# ab28283; RRID: AB_2070798 |
| Mouse monoclonal anti-Muc1 (145Nd) | Biolegend | Cat# 355602; RRID: AB_2561642 |
| Mouse monoclonal anti-Lamp2 (146Nd) | Biolegend | Cat# 354302; RRID: AB_11204245 |
| Mouse monoclonal anti-CDK4 (147Sm) | BD Biosciences | Cat# 559677; RRID: AB_397299 |
| Rabbit monoclonal anti-PTEN (148Nd) | Cell Signaling Technology | Cat# 9559; RRID: AB_390810 |
| Rabbit monoclonal anti-E-Cadherin (149Sm) | Cell Signaling Technology | Cat# 3195; RRID: AB_2291471 |
| Mouse monoclonal anti-Epcam (150Nd) | Biolegend | Cat# 324202; RRID: AB_756076 |
| Mouse monoclonal anti-Her2 (151Eu) | BD Biosciences | Cat# 554299; RRID: AB_395352 |
| Rabbit polyclonal anti-CK5 (152Sm) | Abcam | Cat# ab53121; RRID: AB_869889 |
| Mouse monoclonal anti-CD24 (153Eu) | Biolegend | Cat# 311102; RRID: AB_314851 |
| Mouse monoclonal anti-CDK1 (154Sm) | Biolegend | Cat# 626901; RRID: AB_2074779 |
| Rabbit monoclonal anti-CDK6 (155Gd) | Cell Signaling Technology | Cat# 13331 |
| Rabbit monoclonal anti-p63 (158Gd) | Abcam | Cat# ab124762; RRID: AB_10971840 |
| Rabbit monoclonal anti-TCF7 (159Tb) | Cell Signaling Technology | Cat# 2203; RRID: AB_2199302 |
| Rabbit monoclonal anti-AR (160Gd) | Cell Signaling Technology | Cat# 5153; RRID: AB_10691711 |
| Mouse monoclonal anti-Cyclin A (161Dy) | BD Biosciences | Cat# 554175; RRID: AB_395286 |
| Mouse monoclonal anti-Ki-67 (162Dy) | BD Biosciences | Cat# 550609; RRID: AB_393778 |
| Mouse monoclonal anti-SMA (163Dy) | Thermo Fisher Scientific | Cat# 14–9760-82; RRID: AB_2572996 |
| Mouse monoclonal anti-cPARP (164Dy) | BD Biosciences | Cat# 552596; RRID: AB_394437 |
| Rabbit monoclonal anti-Vimentin (165Ho) | Cell Signaling Technology | Cat# 5741; RRID: AB_10695459 |
| Rat monoclonal anti-GATA-3 (166Er) | eBioscience | Cat# 14–9966-80; RRID: AB_1210520 |
| Rabbit monoclonal anti-p21 (167Er) | Cell Signaling Technology | Cat# 2947; RRID: AB_823586 |
| Rabbit monoclonal anti-phospho-AKT Ser473 (168Er) |
Cell Signaling Technology | Cat# 4060; RRID: AB_2315049 |
| Rabbit monoclonal anti-phospho-STAT3 Tyr705 (169Tm) |
Cell Signaling Technology | Cat# 9145; RRID: AB_2491009 |
| Rabbit monoclonal anti-EGFR (170Er) | Cell Signaling Technology | Cat# 4267; RRID: AB_2246311 |
| Rabbit monoclonal anti-phospho-SMAD2 Ser465/467/Smad3 Ser423/425 (171Yb) |
Cell Signaling Technology | Cat# 8828; RRID: AB_2631089 |
| Rabbit monoclonal anti-ERα (172Yb) | Cell Signaling Technology | Cat# 13258; RRID: AB_2632959 |
| Rat monoclonal anti-CD49f (173Yb) | Biolegend | Cat# 313602; RRID: AB_345296 |
| Rabbit monoclonal anti-phospho-STAT5 Tyr694 (174Yb) |
Cell Signaling Technology | Cat# 4322; RRID: AB_10548756 |
| Rabbit monoclonal anti-phospho-S6 Ser235/236 (175Lu) |
Cell Signaling Technology | Cat# 4858; RRID: AB_916156 |
| Mouse monoclonal anti-CK8/18 (176Yb) | Cell Signaling Technology | Cat# 4546; RRID: AB_2134843 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| C49 (NCGC00371442) | This paper; Johansson et al., 2016 | N/A |
| C70 (NCGC00371443) | This paper; Johansson et al., 2016 | N/A |
| C48 | Genentech | N/A |
| Fulvestrant | Sigma-Aldrich | I4409 |
| 4-hydroxytamoxifen | Sigma-Aldrich | T176 |
| β-Estradiol | Sigma-Aldrich | E2758 |
| GSK126 | Selleckchem | Cat# S7061 |
| Critical Commercial Assays | ||
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | G7573 |
| ThruPLEX DNA-seq 48S Kit | RUBICON | R400427 |
| Deposited Data | ||
| All raw genomic data | GEO | GSE104988 |
| Experimental Models: Cell Lines | ||
| MCF7 cells | Marc Lippman (University of Michigan) | N/A |
| T-47D cells | ATCC | HTB-133 |
| ZR-75–1 cells | ATCC | CRL-1500 |
| BT-474 cells | ATCC | HTB-20 |
| SUM185 cells | Steve Ethier (University of Michigan) | N/A |
| SUM159 cells | Steve Ethier (University of Michigan) | N/A |
| SUM 149 cells | Steve Ethier (University of Michigan) | N/A |
| MDA-MB-231 cells | ATCC | HTB-26 |
| BT549 cells | ATCC | HTB-122 |
| HCC1937 cells | ATCC | CRL-2336 |
| HCC2157 cells | ATCC | CRL-2340 |
| KDM5-C49R | This paper | N/A |
| KDM5-C70R | This paper | N/A |
| FULVR | This paper | N/A |
| TAMR | This paper | N/A |
| MCF7-ESR1Y537S | Myles Brown | N/A |
| MCF7 sgcontrol cells | This paper | N/A |
| MCF7 KDM5B-KO#1 cells | This paper | N/A |
| MCF7 KDM5B-KO#2 cells | This paper | N/A |
| MCF7 KDM5A-KO#1 cells | This paper | N/A |
| MCF7 KDM5A-KO#2 cells | This paper | N/A |
| Recombinant DNA | ||
| Edit-R Lentiviral Blast-Cas9 | Dharmacon | CAS10138 |
| Edit-R Lentiviral sgRNA Non-targeting Control | Dharmacon | GSG11812 |
| Edit-R Human KDM5B Lentiviral sgRNA#1 | Dharmacon | GSGH11838–246552182 |
| Edit-R Human KDM5B Lentiviral sgRNA#2 | Dharmacon | GSGH11838–246552189 |
| Edit-R Human KDM5A Lentiviral sgRNA#1 | Dharmacon | GSGH11838–246592353 |
| Edit-R Human KDM5A Lentiviral sgRNA#2 | Dharmacon | GSGH11838–246592357 |
| siRNA oligos | ||
| ON-TARGETplus Non-targeting siRNA#1 | Dharmacon | D-001810–03-05 |
| ON-TARGETplus Non-targeting siRNA#2 | Dharmacon | D-001810–04-05 |
| ON-TARGETplus Human ESR1 siRNA#1 | Dharmacon | J-003401–11 |
| ON-TARGETplus Human ESR1 siRNA#2 | Dharmacon | J-003401–12 |
| ON-TARGETplus Human ESR1 siRNA#3 | Dharmacon | J-003401–13 |