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. Author manuscript; available in PMC: 2019 Dec 18.
Published in final edited form as: Biomater Sci. 2018 Dec 18;7(1):113–124. doi: 10.1039/c8bm01208c

Figure 2. Functionalization of amph-NPs with whole antibodies or nanobodies.

Figure 2.

(a) Schematic of 3-step process for functionalization of amph-NPs with whole antibodies or VHH nanobodies. (b) SDS-PAGE analysis of anti-CD8 IgG antibody-conjugated amph-NPs (lane 1) and unmodified amph-NPs incubated with antibodies (lane 2). Particles were treated with β-mercaptoethanol to release ligand from the particles prior to PAGE. (c) Quantitative analysis of antibody coupling from SDS-PAGE. (d) SDS-PAGE analysis of anti-CD8 VHH-conjugated amph-NPs (lane 1), unmodified amph-NPs incubated with VHH (lane 2), and unmodified amph-NPs alone (lane 3). Particles were treated with β-mercaptoethanol to release ligand from the particles prior to PAGE.