Skip to main content
. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: J Hepatol. 2018 Jul 18;69(5):1145–1154. doi: 10.1016/j.jhep.2018.07.005

Figure 3: Binge drinking associated with sepsis is characterized by the sustained hepatic presence of neutrophils, NETosis molecules, and increased cell death in mice.

Figure 3:

C57BL/6 mice received three alcohol or sugar gavages with i.p. LPS or saline treatment. Mice were sacrificed after 15h and liver/serum samples analyzed by immunohistology for neutrophil elastase (A), western blot analysis for citrullinated histone H3 (B), RT-qPCR for IL-6 (C) and ELISA for MCP-1(D). TUNEL assay was performed on liver samples from C57BL/6 binge alcohol or sugar gavage mice with or without i.p. LPS or saline treatment as indicated (E). A p < 0.05 compared to baseline sugar gavage (*) or sugar plus i.p. LPS treatment (#) was considered statistically significant using ANOVA for 3–6 mice per experimental group.