Fig 9. SA binding of the NPR1 paralogs.

(a) Purified recombinant proteins were incubated with 4-AzSA, then treated with UV light. 4-AzSA-cross-linked proteins were detected by immunoblot analysis with anti-SA antibody. The first line corresponds to a negative control, where the UV light was omitted. (b) Loading control, the same amount of protein used in A was detected with an anti-MBP. The image was cut to show the MBP empty vector, as indicated by the vertical line.