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Neutralization capability of rabies-specific antibodies in sera from vaccinated and naïve individuals was assessed using RFFIT and HTNT. Based on the complete neutralization of RABV at the 1:5 serum dilution samples were classified as positive or negative. Sensitivity and specificity of HTNT assay for detection of rabies VNAs were calculated based on the RFFIT results and tabulated with 95% confidence interval levels.
