Fig 4. TTV7 in individual whole blood (WB) DNA samples, identified by metagenomic sequencing.

Metagenomic sequencing was applied to the WB DNA samples from the 33 individuals. Reads from Kawasaki disease (KD) patient seq. 1 were mapped to the TTV7 reference genome (a) and showed variants (b). Similarly, the reads from KD patient seq. 5 were mapped to TTV7 (c) and generated variants (d). Although the depth was shallow, the reads covered the entire genome seamlessly (a, c). The variants (b, d) resembled the variants determined by Sanger sequencing (Fig 3), with limited accuracy due to the shallow depth.