Table 1. Nucleotide sequences of polymerase chain reaction primers and conditions for amplification of Anaplasma and Candidatus Neoehrlichia species genes.

Species and target genes	PCR primer sequence (5’-3’)	Annealing (°C/min)	PCR product size (bp)	References
Anaplasma and Ehrlichia spp. groEL (external primer)	GROEL -607F (5′-GAAGATGCWGTWGGWTGTACKGC-3′) GROEL 1294R (5′-AGMGCTTCWCCTTCWACRTCYTC-3′)	57	688	30
Anaplasma and Ehrlichia spp. groEL (internal primer)	GROEL- 667F (5′-ATTACTCAGAGTGCTTCTCARTG-3′) GROEL -1121R (5′-TGCATACCRTCAGTYTTTTCAAC-3′)	57	445	30
Ca. Neoehrlichia 16S rRNA (external primer)	16S-EC9-F (5′-TACCTTGTTACGACTT-3′) 16S-EC12A-R (5′-TGATCCTGGCTCAGAACGAACG-3′)	41	1,462	5
Ca. Neoehrlichia 16S rRNA (internal primer)	16S-IS58-62f (5′-GGAATAGCTGTTAGAAATGACA-3′) 16S-IS58-594r (5′-CTATCCTCTCTCGATCTCTAGTTT-3′)	54	488	5
A. phagocytophilum 16S rRNA (external primer)	AE1-F (5′-AAGCTTAACACATGCAAGTCGAA-3′) AE1-R (5′-AGTCACTGA CCCAACCTTAAATG-3′)	56	1,406	30
A. phagocytophilum 16S rRNA (internal primer)	AP-F (5′-GTCGAACGGATTATTCTTTATAGCTTGC-3′) AP-R (5′-CCCTTCCGTTAAGAAGGATCTAATCTCC-3′)	56	926	30