(
A) Inactive dimer of the full-length MnmE in the GTP-bound form (the structure (PDB: 3GEI) was resolved with non-hydrolyzable GTP analogs). The P-loop domain is shown in grey, the K-loop is not resolved (its position is indicated by red asterisks), the N-terminal and helical domains are shown in blue and green for different monomers. (
B) An active dimer of isolated G-domains of MnmE, as resolved in complex with a transition state analog and K
+ ion (PDB: 2GJ8). The K-loops are shown in red, K
+ ions are shown as purple spheres. (
C) Schematic representation of the conformational changes in MnmE dimers, reproduced after (
Klare, 2013), domains are colored the same way as on panel A.