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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Methods Mol Biol. 2013;1042:47–60. doi: 10.1007/978-1-62703-526-2_4

Figure 2.

Figure 2.

Visualization of nascent RNA in living cells. A) DIC image of U2-OS cells containing a reporter gene inserted randomly into the genome. Nucleoli are visible as dense bodies within the nucleus. B) Fluorescence image of PP7-mCherry coat protein with a nuclear localization signal. The coat protein accumulates in the nucleus. The nascent transcription site is visible as a punctate spot (white arrows). At high coat protein expression levels, one frequently observes nucleolus staining as well. The box indicates the region which is magnified in panel D. C) Merge of panels A, B. Transcription sites are indicated with white arrows. D) Magnification of the demarcated region in panel B. The transcription site is visible near the edge of the nucleus.