Fig. 7.

Line tension increases as ρ increases and Ld + Lo domains become visible at higher line tension at three Chol fractions of 0.27, 0.3, and 0.33 for BSM/DOPC/POPC/Chol.

(A) In all three lipid mixtures, visible domains first appear at line tension between 0.1 to 0.3 pN (dotted line). Modulated GUVs become dominant at line tension between 0.3 to 0.5 pN (dashed line). Above 0.5 pN, macroscopic GUVs with large and round domains account for the majority (long dashed line). Line tension values from 5 to 15 GUV domains were determined and averaged for each data point at different ρ values; (B) Fractions of visible lipid domains (modulated plus macro domains) correspond to line tension change as ρ increases. Error bars correspond to SE.