Skip to main content
. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Neurochem. 2018 Nov 19;147(6):831–848. doi: 10.1111/jnc.14580

Figure 1. Opposing effects of Mn and FAC exposure to cell viability and protein expression of APP and H-Ferritin in human neuroblastoma cells.

Figure 1.

Human SH-SY5Y cells were incubated with increasing Mn concentrations for 24 h and 48 h and analyzed for (a) cell viability (n=3) and (b) cell number (24 h, n=6; 48 h, n=4). (c) Cell lysates of control and Mn treated SH-SY5Y cells were separated on 4-12% Bis-Tris gels. Western blot and densitometric quantification of APP (clone A8717) and H-Ferritin (clone D1D4) (n=3). (d) SH-SY5Y cells treated with increasing FAC concentrations for 24 h, immunoblotted for APP (clone A8717) and H-Ferritin (clone D1D4). Densitometric quantification of both proteins were adjusted to loading control β-actin (n=3). Data shown represent mean ± SD. N number indicates number of replicates. Differences were calculated using either one-way ANOVA followed by Bonferroni post hoc analyses (a, b) or unpaired t-test (c, d) (*, p < 0.05; **, p < 0.01; ***, p < 0.001).