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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Neurochem. 2018 Nov 19;147(6):831–848. doi: 10.1111/jnc.14580

Figure 2. Acute exposures of Mn and FAC differentially impacts protein and mRNA levels of APP and H-Ferritin.

Figure 2.

(a) SH-SY5Y cells treated with 100 µM Mn for indicated time points and immunoblotted for APP (clone 22C11) and H-Ferritin (clone D1D4). All densitometric analyses were standardized to corresponding β-actin levels (n=3). (b) SH-SY5Y cells were incubated with 100 µM FAC for indicated time points and immunoblotted for APP (clone 22C11) and H-Ferritin (clone D1D4). All densitometric analyses were standardized to corresponding β-actin levels (n=3). (c) RT-PCR analysis of indicated iron responsive target genes (APP, H-Ferritin, TFR1 and FPN) from SH-SY5Y cells incubated for 24 h with 100 µM FAC or 100µM Mn. All data are normalized to housekeeping gene β-actin and presented as relative fold-induction compared to untreated controls (n=4). RT-PCR analysis of DFO-treated SH-SY5Y cells were shown in Supplemental Fig 1. Data shown represent mean ± SD. N number indicates number of replicates. Differences were calculated using unpaired t-test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).