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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Neurochem. 2018 Nov 19;147(6):831–848. doi: 10.1111/jnc.14580

Figure 5: APP over-expression protects against Mn-induced toxicity via attenuating oxidative stress.

Figure 5:

(a) Representative histogram of cellular ROS (left panel) and lipid ROS (right panel) levels of SH-SY5Y cells, either stably overexpressing the APP695-isoform (APP695) or with the empty vector pCEP4 (Mock). (b) SH-SY5Y Mock and APP695 cells were exposed to Mn for 24 h and analyzed for cellular oxidative cells via DCF dye (left panel) or lipid-ROS using BODIPY-C11 (right panel). DCF fluorescence is presented as arbitrary fluorescence units (F.U) (n=3). Lipid-ROS data are presented as FL1/FL2 ratio (n=3). (c) Mock and APP695-transfected SH-SY5Y cells were incubated with increasing Mn concentrations for 24 h and analyzed for APP (clone 22C11) and H-Ferritin (clone D1D4) protein expression. (d) Cell viability was analyzed in Mock and APP695-expressing SH-SY5Y cells after Mn exposure for 24 h (n=3). All other data are presented as mean ± SD. N number indicates number of replicates. Differences were calculated using two-way ANOVA (*, p < 0.05; **, p < 0.01; ***,p < 0.001).