Figure 2.

Short Duration Spikes to High Temperature Affect VIN3 Expression

(A) Temperature conditions given daily for 4 weeks (left) and then on day of sampling (right). Plants were grown in 20°C (night) or 22°C (day) 16-hr photoperiod for 1 week and then transferred to the conditions shown on the left. Dark background indicates nighttime (8-hr photoperiod).

(B) VIN3 spliced expression during the day of sampling, sampled every 3 hr over a 12-hr period as shown. The green background indicates the time of the high temperature spike in the midday spike conditions. n = 1–9; average > 6.

(C) VIN3 unspliced expression from experiment in (B). n = 1–9; average > 6.

(D) VIN3 expression after 4 weeks cold in indicated conditions. “Before” refers to samples taken at 18:30 on sampling day, in the conditions indicated. “After” refers to samples that after 4 weeks cold in indicated conditions were further treated with, first, a further 4 days in the conditions indicated and then transferred in the afternoon (before dark) to constant 8°C conditions for approximately 24 hr before sampling at 18:30. n = 2–8; average = 4.4.

(E) FLC expression averaged over all the time points of sampling day after 4 weeks cold. Kruskal-Wallis with Dunn’s post hoc test between midday spike, night spike and spike memory (conditions with similar VIN3 expression for the 4 weeks of the treatment to test for VIN3-independent effect only) gives $\text{p}<0.05$ significant difference (^∗ in plot) between night spike and midday spike and between night spike and spike memory (no significant difference between midday spike and spike memory). Boxplots show median and 25^th and 75^th percentiles of the samples. Ends of whiskers show maximum and minimum values. n = 12–38; average > 30. In all cases, circle and bars show mean and standard error, respectively. RNA levels normalized to UBC, PP2A.

See also Figures S3 and S4.