Fig. 5.

Kinetics of (A) the SLD translator and (B) the DLD translator with/without the input $X$. The leak rate in the SLD design increases with larger temperature (at 37 °C, leak rates were measured at three different time windows), while the leak in the DLD design was not measurable in this experimental setup. The desired triggering signal with the input strand $X$ reaches more than $75\%$ completion before the first measurable data point. [Because the samples are otherwise identical, we assume that, before addition of the trigger, the triggered samples had initial fluorescence levels no higher than the untriggered samples. A slight decrease in the SLD signal with input at 37 °C during the first hour appears in some experiments (SI Appendix, Fig. S1A).] Species concentration: [reporter] = 400 nM; [fuels] = 350 nM; [input $X$] = 350 nM. Time 0 represents the moment that the first data point was measured; the inputs are added no more than 3 min before measurement at 25 °C and no more than 13 min before measurement at 30 °C and 37 °C.