Fig. 8.

DLD linear translator cascade. (A) Scheme of a four-layer translator cascade with nine steps of strand displacement implemented with eight fuel complexes and a reporter. (B) Kinetics of the desired reactions in the presence of input and the leak reactions in the absence of input. We tested different depths of the cascade by including a different subset of fuels. In each case, the desired reaction reached half-completion at the first measured data point. In the timeframe of 100 min, the leak fraction ([leak]/[initial fuel]) is less than 4%. (Inset) Leak of the translator cascade over 10 h. The largest leak fraction is roughly 5%: [reporter] = [fuels] = 500 nM, [input] = 250 nM, 25 °C. The inputs are added no more than 3 min before measurement.