Fig. 5.
PT2399 treatment reduces fatty liver symptoms only in WT mice fed HFD. WT and KI mice fed HFD for 12 wk were administered vehicle or 30 mg/kg PT2399 twice a day for the final 8 wk. HE staining of liver tissues (A) and liver TG levels (B) from WT and KI mice. (Magnification: A, 200×.) (C) Relative liver DAG and TAG levels in WT and KI mice (n = 4 per group). (D) qPCR analysis of lipid metabolism genes in the liver. (E) Serum TG levels of WT mice. (F) TG clearance of WT mice following 7 wk of PT2399 treatment. (G) Serum ALT levels from WT or KI mice. n = 8 per group. Data are presented as mean ± SEM. Significance between every two groups was calculated using unpaired two-tailed Student’s t test. Two-way ANOVA was performed for comparison of two groups over time followed by Sidak’s multiple test. *P < 0.05, **P < 0.01.