Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 11;115(52):E12285–E12294. doi: 10.1073/pnas.1809682115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published under the PNAS license.

PMC Copyright notice

Fig. 4. — Thermoregulation in APE1 mice is associated with a loss of serotonergic neurons. (A) A cartoon depiction of the thermoregulatory DR and RMR nuclei. (B) Nissl staining of the DR and RMR shows decreased cellularity in the Ape1^Nes-cre tissue compared with control. Arrows indicate areas of decreased cellularity. Aq, cerebral aqueduct. (C) Identification of serotonergic neurons in the DR nuclei using TPH2 shows a decrease in TPH2⁺ neurons in Ape1^Nes-cre tissue compared with control tissue; arrows indicate TPH2⁺ cells. (D) TPH2⁺ neurons are reduced in number in Ape1^Nes-cre tissue compared with control in both the DR (∼25% reduction) and the RMR (50% reduction). ***P < 0.001. (E and F) Colocalization of TPH2 and NeuN immunostaining in the DR (E) and the RMR (F) shows that reduction of TPH2 is due to the loss of neurons. (G) Reduced numbers of vGluT3 neurons (arrows) are also found in Ape1^Nes-cre tissue compared with control tissue.