Fig. 3.

HNF4A targets CLOCK:BMAL1 differently from CRY1. (A and B) HNF4A is able to suppress a CLOCK:BMAL1 mutant which interferes with sequestration by CRY1. HEK 293T cells were cotransfected with reporter gene Per1-Luc, wild-type or mutant CLOCK:BMAL1, and increasing amounts of CRY1 (A) or HNF4A (B) plasmid (n = 3 for each condition, mean ± SD). Percentages on the Top indicate the degree of CLOCK:BMAL1 repression.