Fig. 1.

lmo0762 is an antibiotic resistance gene. (A) RNA-seq profile of rli80-lmo0762 locus obtained from L. monocytogenes grown in absence (black) or in presence of lincomycin (0.5 µg/mL, green). (B) Western blot analysis performed on WT (no Flag) and a strain expressing Lmo0762 with a Flag at the C terminus (Flag). EF-Tu was used as a loading control. (C) Schematic representation of MIC assay. The colors indicate that the tested strain is more sensitive (blue) or more resistant (red) to the antibiotic compared with the reference (ref) strain. The MIC values are indicated in the boxes after 48-h incubation (in μg/mL). (D) Induction of lmo0762 and lmo1296 upon antibiotic treatment calculated by qPCR in comparison with the endogenous level before addition of the antibiotic. Data are represented as mean ± SEM. We used a one-way ANOVA on ΔCt values for statistics, using biological replicates as pairing factors. *P < 0.05, **P < 0.01; ns, nonsignificant.