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. 2018 Dec 12;115(52):E12313–E12322. doi: 10.1073/pnas.1814705115

Fig. 4.

Fig. 4.

p38α deficiency in cDC1s, but not cDC2s, promotes Tr1 generation by up-regulating IL-27 expression. (A and B) Colonic DCs of wild-type (WT) and p38αΔDC mice were cocultured with naïve CD4+ T cells for 6 d, and then, IL-10 secretion (n = 3; A) and Tr1 differentiation (n = 3; B) were analyzed. (C and D) Colonic cDC1s and cDC2s of WT and p38αΔDC mice were cocultured with naïve CD4+ T cells for 6 d to examine Tr1-related gene expression (n = 5; C) or IL-27 secretion (n = 3; D). (E) Colonic cDC1s of WT and p38αΔDC mice were cocultured with naïve CD4+ T cells with αIL-27 or isotype for 6 d to check Tr1 differentiation (n = 3). (F and G) WT, p38αΔDC, IL-27ΔDC, and DKO (both p38α and IL-27 were deleted in DCs) mice were supplied with 7 d of 3% DSS solution followed with normal drinking water (n = 6). Body weight (F) and Tr1 differentiation in colon tissue (G) were examined. Data represent mean ± SEM. Two-tailed Student’s t test (A and B), two-way ANOVA with Bonferroni posttest (C–F), and one-way ANOVA with Tukey’s posttest (G). Results were replicated (n ≥ 2 experiments). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.