Fig. 2.

MIM^EX15-mutant Purkinje neurons undergo autophagy. (A) MIM^EX15 mutants display fused mitochondria shown by increased complex 5 ATP-synthase immunostaining and collapsed Golgi shown by reduced giantin immunostaining at 4 wk. (B) Eight-week-old MIM^EX15 mutants show increased LC3-II abundance. *P < 0.005; Student’s t test. (C) MIM^EX15 mutants show increased levels of mRNA for the autophagocytic marker VMP1. *P < 0.05; Student’s t test. (D) MIM^EX15 mutants show increased microglial infiltration shown by Aif1 transcript. *P < 0.002; Student’s t test. (E) MIM^EX15 mutants show GFAP⁺ glial infiltration during disease progression. (F) Western blots quantifying increased cerebellar GFAP. *P < 0.05; Student’s t test. (G) MIM^EX15-mutant cerebella do not have increased TUNEL staining at 4, 8, or 16 wk of age.