Table 1.

Effect of acid treatments on the conformational states of EV-D68 strain MO

Dataset	A_Acid_1	A_Acid_2	A_Acid_3	A_Acid_4
Temperature	4 °C	RT*	RT*	RT*
Duration†	3 min	3 min	30 s	3 min
pH	6.5	6.5	6.0	6.0
No. of full particles	32,972	68,112	100,113	19,798
No. of empty particles	891	2,389	4,687	184
Percentage of full particles	97.4	96.6	95.5	99.1
Percentage of full native virions‡	76.6 ± 0.4	40.2 ± 0.2	37.4 ± 0.4	3.5 ± 0.1
Percentage of A particles‡	7.3 ± 0.2	56.4 ± 0.2	58.1 ± 0.4	95.6 ± 0.1
Percentage of E1 particles‡	13.5 ± 0.5	—§	—§	—§

^*Room temperature.

^†Approximate time from the initiation of acid treatment to the state of being quenched by freezing.

^‡Percentage among all particles. Data are presented as mean ± SD; 3D classifications were performed six times.

^§Not identified.