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. 2018 Dec 15;7(12):bio036517. doi: 10.1242/bio.036517

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Gata4^H2B-GFP/+ reporter expression at E8.5. Left panels show lateral whole-mount Gata4^H2B-GFP/+ embryos at E8.5 prior to fixation with GFP overlaid over bright field or GFP alone. (A,B) Transverse sections of whole-mount stained Gata4^H2B-GFP/+ embryos for endogenous GATA4 protein. GFP was visualised directly and DNA was stained with Hoechst at positions indicated on whole-mount embryo. (A,A′) GFP expression is observed throughout the endocardium (inner) and myocardium (outer) layers of the heart and is co-expressed with GATA4 protein (B,B′) and also in the sinus venosus, septum transversum (st) and yolk sac (YS) endoderm. Scale bars: 100 μm. Neural tube (NT), foregut (fg), right (R), left (L), dorsal (D), ventral (V), anterior (A), posterior (P), right ventricle (rv), left ventricle (lv). Arrowheads indicate myocardial wall and asterisk indicates endocardium.