Fig. 4.

Effects of EB3 depletion in Vero cells. (A) Life histories of single MTs in 3T3 cells transfected with alpha-tubulin, MTs in untreated cells demonstrate dynamic instability. (B) Life histories of MTs in 3T3 cells, expressing shEB3 and transfected with alpha-tubulin. In EB3-depleted cells dynamic instability of MTs was suppressed. (C–D) Immunofluorescent staining with EB3 antibodies, in EB3-depleted cells (D) comets are almost absent compared to control cells (C). (E) Spreading of Vero cells in normal conditions (white circles), in EB3-depleted cells (black circles) and in cells with stabilized MTs (black triangles). (F) Spreading of EB3-depleted cell is mainly anisotropic, the cell fails to form a large lamellum and spreads through formation of short lamellipodia. Scale bars: 10 µm.