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. 2018 Dec 15;7(12):bio036194. doi: 10.1242/bio.036194

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — The verification of the BMSCs and the change of IRS-1, TAZ, RUNX2 and OCN mRNA expression during osteogenic differentiation. (A) The BMSCs were isolated from bone marrow, cultured in vitrol and verified by flow cytometry for hematopoietic surface markers CD34 (0.6%) and CD45 (1.6%), and BMSC surface markers CD29 (100%) and CD90 (96%). (B,C) Passage 3 BMSCs were cultured with growth medium or (C) osteogenic medium for 14 days. (D) Alizarin Red staining was performed and (E) the quantification by cetylpyridinium chloride dissolution was presented after inducing osteogenic medium for 14 days. (F) The mRNA expression of IRS-1, TAZ, RUNX2 and CON in BMSCs cultured in osteogenic medium for 3, 7 and 14 days were measured by fluorogenic quantitative PCR. (s.d.±mean; n=3) *P<0.05 versus control group.