Fig. 3.

SiIRS-1 transfection inhibited osteogenic differentiation as well as decreasing TAZ expression. (A-G) SiIRS-1 transfection inhibited the expression of TAZ, RUNX2 and OCN; measured by fluorogenic quantitative PCR and western blot. (H) The ALP activities were measured at 0, 3 and 7 days after osteogenic medium introduction. (I,J) Alizarin Red staining was performed and the normalized Alizarin Red absorption was presented after inducing osteogenic medium for 14 days. (s.d.±mean; n=3) *P<0.05 versus non-transfected group; ^#P<0.05 versus negative control group.