Fig. 5.

Compounds D14 and C22 induce apoptosis in the ondependent cell line of KRas4B. a, b and c Cell death of MIA PaCa-2 and hTERT-HPNE cells was determined by apoxin V / 7-AAD / CytoCalcein Violet and analyzed by flow cytometry. d Compounds D14 and C22 induced apoptosis by being reflected in the presence of the phosphorylation of P53, Cytochrome-c, procaspase 3, and Smac/Diablo on the MIA PaCa-2 cell line of KRas4B-deoendent. The phosphorylation of these proteins was significantly compared with the control. The total protein extract (300 μg) was used for the apootosis kit. The dots of the matrix were visualized according to the manufacturer’s instructions. The intensity of each point was measured as described in “Material and methods”. The upper panel provides matrix analysis of MIA PaCa-2 without compounds (control); the central panel shows the matrix analysis of the MIA PaCa-2 cell line treated with compound D14; the lower panel shows the matrix analysis of the MIA PaCa-2 cell line treated with the C22 compulsion. e Normalized quantification graph shows the relative change of the phosphorylation differences with respect to the control