Fig. 5.

Usage of splice sites. Usage of splice sites was calculated based on the number of RNA-Seq reads supporting the exon next to a splice site and the number of reads supporting the intron containing the splice site. There is a substantial difference between the usage of 5′ and 3′ splice sites in favor of the 5′ splice sites. Canonical GT-AG splice site combinations are used more often than major or minor non-canonical splice site combinations. Sample size (n) and median (m) of the usage values are given for all splice sites