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. 2018 Dec 29;18:377. doi: 10.1186/s12870-018-1594-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PCR amplification of Ganoderma species DNA using specific primer pairs of Ganoderma species. Lane C: uninoculated control; Lane T1D3: normal PCR for 3 d.p.i; Lane T1D7n: nested PCR for 7 d.p.i; lane T1D11n: nested PCR for 11 d.p.i; +VE: normal PCR for pure Ganoderma culture. The amplicon size for T1D3 and + VE are 223 bp whilst T1D7n and T1D11n amplicon size are 102 bp