Table 2.
Methylation summary of whole genome bisulphite sequencing
| Normal mucosa (n = 5) | Adenoma (n = 4) | |
|---|---|---|
| Nuclear Genome | ||
| CG methylation % | 74.5 ± 0.50 | 67.9 ± 1.16* |
| CHG methylation % | 1.3 ± 0.06 | 1.0 ± 0.05 |
| CHH methylation % | 1.3 ± 0.07 | 1.0 ± 0.05 |
| Mitochondrial Genome | ||
| CG methylation % | 1.0 ± 0.09 | 1.0 ± 0.16 |
| CHG methylation % | 1.3 ± 0.14 | 0.8 ± 0.14 |
| CHH methylation % | 1.2 ± 0.06 | 0.92 ± 0.12 |
| Lambda control % unconverted cytosines | 1.06 ± 0.06 | 0.86 ± 0.05 |
Average methylation percentage ± SEM, *P < 0.01 Normal vs Adenoma. No statistical tests were performed on methylation levels within the range of the lambda controls (which indicate conversion efficiency)