Fig. 4.

Expression of biologically-active human CSF1-Fc by transgenic hens. a Purity of 10 μL samples from 15 mL size exclusion fractions of hCSF1-Fc from egg white demonstrated by non-reducing SDS-PAGE stained with Instant Blue. Identity was confirmed by western blot transfer and staining with mouse anti-human CSF1 antibody and LICOR IRDye 680RD Donkey anti-Mouse IgG (H + L) secondary antibody. b Western blot of 10 μL samples from each stage of purification: MabSelect SuRe load (lane 1), MabSelect SuRe flow-through (lane 2), MabSelect SuRe wash (lane 3), MabSelect SuRe eluate (lane 4) and final pure protein (lane 5). c Bone marrow from pigs was cultured in purified pCSF1-Fc from egg white, purified hCSF1-Fc from egg white, and the same protein after vacuum drying and reconstitution, or without growth factor (cells only) for 7 days. MTT was used to assess cell viability. Measurements were taken in triplicate. Graph shows mean + SEM