Table 3.

Profile of oncogenic PIK3CA mutations in the three study groups

Group	Sample ID	Exon	Codon	Codon (WT – MUT)	Type of mutation	Amino acids change	Nature of mutation	COSMIC ID
CM/ESCC	111 T	9	545	GAG – GCA	A → C	E545A	Missense	COSM12458
	122 T	9	549	GAT – CAT	G → C	D549H	Missense	COSM219119
	114 T	20	1027	GCC – GAC	C → A	A1027D	Missense	Not reported
	119 T	20	1047	CAT – CTT	A → T	H1047L	Missense	COSM776
	124 T	20	1047	CAT–CGT	A → G	H1047R	Missense	COSM775
ESCC	26 T	9	545	GAG – GCG	A → C	E545A	Missense	COSM12458
	120 T	9	555	AGG – AAG	G → A	R555K	Missense	COSM1716158
	36 T	9	524	AGG – AAG	G → A	R524K	Missense	COSM53245
	4 T	20	1030	AAA – AGA	A → G	K1030R	Missense	Not reported
CME	101 M	20	1053	ACA – AAA	C → A	T1053K	Missense	Not reported

CME/ESCC – chagasic megaesophagus associated with esophageal squamous cell carcinoma; ESCC – esophageal squamous cell carcinoma without chagasic megaesophagus; CME – chagasic megaesophagus without esophageal squamous cell carcinoma. A – adenine; C – cytosine; G – guanine; T – thymine. WT – wild-type; MUT – mutant