. Author manuscript; available in PMC: 2018 Dec 30.

Published in final edited form as: Cell Syst. 2017 Nov 1;5(5):518–526.e3. doi: 10.1016/j.cels.2017.10.004

Table 1: Parameters for the allosteric sigmoidal fit of adsorption of SpoVM^WT and SpoVM variants onto differently curved surfaces as measured by flow cytometry.

B_max, maximal binding value; h, Hill coefficient; K_1/2, concentration of protein producing half-maximal binding; MESF, molecules of equivalent soluble fluorochrome. Errors are SEM (n=3 independent trials, each trial containing data from >30,000 SSLBs). “N/D” indicates “not determinable”.

Protein	Bead diam. (μm)	B_max (MESF/μm²)	h	K_1/2 (μM)
SpoVM-FITC	2	3,920 (± 100)	4.3 (± 0.8)	0.58 (± 0.03)
	8	4,450 (± 200)	2.2 (± 0.3)	1.0 (± 0.07)
SpoVM^P9A-FITC	2	3,040 (± 70)	1.5 (± 0.1)	0.58 (± 0.03)
	8	3,340 (± 480)	1.1 (± 0.3)	0.84 (± 0.26)
SpoVM^L8P,P9A-FITC	2	3,640 (± 190)	1.9 (± 0.2)	1.67 (± 0.13)
	8	5,140 (± 750)	2.4 (± 0.4)	3.40 (± 0.53)
SpoVM^P9A,K10P-FITC	2	4,350 (± 310)	1.8 (± 0.2)	1.81 (± 0.20)
	8	N/D	1.1 (± 0.3)	N/D
SpoVM^P9A,F11P-FITC	2	4,150 (± 160)	2.5 (± 0.3)	1.48 (± 0.07)
	8	N/D	1.2 (± 0.2)	N/D
SpoVM^P9G-FITC	2	2,950 (± 80)	3.9 (± 0.9)	0.41 (± 0.03)
	8	3,090 (± 560)	1.1 (± 0.4)	0.83 (± 0.33)

Table 1: Parameters for the allosteric sigmoidal fit of adsorption of SpoVMWT and SpoVM variants onto differently curved surfaces as measured by flow cytometry.

Table 1: Parameters for the allosteric sigmoidal fit of adsorption of SpoVM^WT and SpoVM variants onto differently curved surfaces as measured by flow cytometry.