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. Author manuscript; available in PMC: 2018 Dec 30.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Aug 15;56(40):12052–12063. doi: 10.1002/anie.201701065

Figure 13.

Figure 13

Feedback in splicing modulation. a) RT-PCR and b) qRT-PCR analysis of MCL-B cells treated with 100 nm 1, 3, or 24, or a DMSO control for 4 h. c) Pladienolide B (1) regulates the level of SF3B1 phosphorylation. JeKo-1 cells were treated with 100 nm 1 for 6 h, 12 h or 24 h. Untreated cells grown for 24 h were used as a control (C). d) Schematic representation of the feedback modulation of SF3B1. Inhibition of SF3B1 (green) results in IR in SF3B1 and leads to a reduction in the amount of SF3B1 protein (orange) within the U2 snRNP. The net effect is a reduction in SF3B1 levels and the formation of a compromised splice-altered U2 snRNP.