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. Author manuscript; available in PMC: 2018 Dec 30.
Published in final edited form as: Hepatology. 2015 Feb;61(2):574–584. doi: 10.1002/hep.27543

Fig. 5.

Fig. 5.

Expression of hepatic cancer stem cell-related proteins in HCC tissues related to HCC prognosis in validation study. (A,B) Hematoxylin & eosin and hepatic cancer stem cell-related protein immunostaining of stem cell-like subtype HCCs (A) and metabolic subtype HCCs (B) from the 227-case validation dataset. The staining images show high expression of ACVR1, CD44, EPCAM, CD24, CD90, and IL-8 in the stem celllike subtype HCC tissues (A) and low expression of these proteins in metabolic subtype HCC tissues (left panel: magnification ×40; right panel: magnification ×400). (C,D) Kaplan-Meier analysis of overall survival (C) and recurrence-free survival (D) according to expression of hepatic cancer stem cell-related proteins in the validation set. The expression of ACVR1, CD44, CD24, and CD90 was significantly associated with poor overall survival and recurrence-free survival. The expression of EPCAM and IL-8 was significantly associated with poor overall survival but not with recurrence-free survival. Red lines represent positive for protein markers, and blue lines represent negative for protein markers.