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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2018 Oct 2;1866(3):395–408. doi: 10.1016/j.bbamcr.2018.10.001

Figure 3: Loss of ADD3 increases transfilter migration and Matrigel invasion of lung cancer cells.

Figure 3:

ADD3 expression was down-regulated HI573 cells using CRISPR/Cas9-mediated gene editing. (A) Immunoblotting analysis shows co-depletion of ADD3 and ADD1 by 4 different ADD3 small guide (sg) RNAs. (B) Quantification of the planar migration of the control and ADD3-depleted HI573 cell monolayers at 24 h post-wounding. (C, D) Representative images and quantitative analysis of the DAPI-labeled control and ADD3-deficient HI 573 cells after 16 h of transfilter migration in the Boyden chamber. (E, F) Representative images and quantitative analysis of the DAPI-labeled control and ADD3-deficient HI573 cells after 24 h invasion into Matrigel. Data are presented as mean ± SE (n =3); *p < 0.05; **p < 0.005, as compared to the control sgRNA-transfected group.