Skip to main content
. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2018 Oct 2;1866(3):395–408. doi: 10.1016/j.bbamcr.2018.10.001

Figure 7: Downregulation of Src expression reverses the increased adhesion and attenuated motility of ADDl-overexpressing lung cancer cells.

Figure 7:

Control and ADD 1-overexpressing H1299 cells were transfected with either Src or non-targeted siRNAs and were analyzed on day 3 posttransfection. (A) Immunoblotting analysis shows the expression of Src and p-paxillin in the control and ADDl-overexpressing cells transfected with either non-targeting or Src-specific siRNAs. (B) Immunolabeling analysis of NM IIB in ADDl-overexpressing lung cancer cells transfected with either control or Src-specific siRNAs. Arrows show long NM IIB-rich protrusions in ADD1-overexpressing cells that disappear after Src depletion. (C, D) Representative images and quantification of collagen I matrix adhesion of the control and ADD 1-overexpressing H1299 cells transfected with either control or Src-specific siRNAs. (E, F) Representative images and quantification analysis of transfilter migration of control and ADD 1-overexpressing H1299 cells transfected with either control or Src-specific siRNAs. Data are presented as mean ± SE (n = 3); **p < 0.005.