Figure 2. Accumulation of Cpd-1 and Cpd-2 depends on LamB expression.

The strains were grown in different media to control the expression of the LamB porin and the MalE transporter; 2 components of the maltose regulon (see Figs S2 and S3). See Fig S1 for the characteristics and the corresponding immunoblots of the strains. (A) Presence of LamB and MalE by Western blot in RAM1292 and RAM2808 under different growth conditions. (B, C) Number of Cpd-1 (B) and Cpd-2 (C) molecules accumulated per cell in the various studied strains following analysis by spectrofluorimetry. The columns with bars (SDs) correspond to measurements carried out in triplicate. Calibration curves were used to obtain the number of molecules per cell (Fig S4). (D) Microfluorimetric images obtained with DUV microscopy with pellets of RAM2808 with or without induction of the LamB porin incubated without and with Cpd-1 or Cpd-2. Controls are RAM2808 cells incubated without Cpd-1 and Cpd-2. (E) Microfluorimetric results obtained from (D). Data are represented with a box-and-whisker plots, which is a way of summarizing the essential profile of a quantitative statistical series: the boxes represent data-points from the 25th to 75th percentiles; the middle horizontal lines represent the median data point and the whiskers show the span of the data for each sample. The outliers are represented by red + signs.