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. 2018 Dec 28;2(1):e201800242. doi: 10.26508/lsa.201800242

Figure 3. Time-course of accumulation of Cpd-1 and Cpd-2 in RAM2808 and RAM1292 with repression or induction of the maltose operon.

Figure 3.

(A, B) Time-course accumulation in RAM2808 cells grown without or with 0.2% arabinose (Ara, induction of the LamB expression), 0.4% maltose, and 0.2% arabinose (Malt Ara, induction of the maltose operon and LamB expression); and incubated with Cpd-1 (A) or Cpd-2 (B). The columns with bars (SDs) corresponded to measurements carried out in triplicate. (C, D) Calibration curves of the fluorescence of Cpd-1 (C) and Cpd-2 (D) used to obtain the number of molecules per cell shown in (A) and (B). (E) Time-course accumulation measured with DUV microspectrofluorimetry in RAM1292 cells grown with 0.4% glucose (Glc, repression of the maltose operon, dotted lines) or 0.4% maltose (malt, induction of the maltose operon, full lines). Cell pellets were resuspended extemporaneously under DUV microscope without (black lines) or with Cpd-1 (green lines) or Cpd-2 (purple lines). Insert: Enlargement of the Cpd-2 results.