Skip to main content
. 2018 Dec 28;2(1):e201800242. doi: 10.26508/lsa.201800242

Figure 5. Detection of Cpd-1 in the different cell compartments of RAM1292.

Figure 5.

The RAM1292 cells were grown in the presence of 0.4% maltose and incubated with Cpd-1. Samples were recovered at various time points and a fractionation protocol was performed to obtain the total (TOT, periplasm + spheroplast), periplasmic (PERI), cytoplasmic (CYTO), and membrane (MB) fractions of the cells. Fluorospectrometry measurements were performed to determine the levels of Cpd-1 in each fraction. Note: Western blots were performed to confirm the distribution of specific proteins of the different cellular fractions (Fig S8A). Calibration curves were used to obtain the number of molecules per cell and per compartment (Fig S8B).